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Table 2 Identification of SSST blind samples. MIRL22-A.unknown-24 to 86 were provided by the Saskatoon Seed Science and Technology unit. The samples corresponding to MIRL22-A.unknown-37, 49 and 71 were not included due to lack of valid morphological identification

From: Seed protein biotyping in Amaranthus species: a tool for rapid identification of weedy amaranths of concern

Sample Name

Identification Call1

Average Bruker score2

Original phenotypic Identification3

MIRL22-A.unknown-24

Amaranthus spinosus

1.30

Amaranthus palmeri atypical5

MIRL22-A.unknown-25

Amaranthus retroflexus

2.47

Amaranthus retroflexus

MIRL22-A.unknown-26

Amaranthus tricolor

1.92

Amaranthus tricolor

MIRL22-A.unknown-27

Amaranthus powelli

2.14

Amaranthus powellii subsp. powellii

MIRL22-A.unknown-28

Amaranthus caudatus

2.31

Amaranthus caudatus

MIRL22-A.unknown-29

Amaranthus palmeri

2.11

Amaranthus palmeri

MIRL22-A.unknown-30

Amaranthus arenicola

1.95

Amaranthus arenicola

MIRL22-A.unknown-31

Amaranthus albus

2.04

Amaranthus albus

MIRL22-A.unknown-32

Amaranthus tuberculatus

2.16

Amaranthus tuberculatus atypical

MIRL22-A.unknown-33

Amaranthus californicus

2.25

Amaranthus californicus

MIRL22-A.unknown-34

Amaranthus californicus

2.26

Amaranthus californicus

MIRL22-A.unknown-35

Amaranthus spinosus

2.24

Amaranthus spinosus

MIRL22-A.unknown-36

Amaranthus caudatus

2.34

Amaranthus caudatus

MIRL22-A.unknown-38

Amaranthus tuberculatus

2.09

Amaranthus tuberculatus

MIRL22-A.unknown-39

Amaranthus albus

1.92

Amaranthus albus

MIRL22-A.unknown-40

Amaranthus californicus

2.30

Amaranthus californicus

MIRL22-A.unknown-41

Amaranthus powelli

2.16

Amaranthus powellii

MIRL22-A.unknown-42

Amaranthus albus

2.19

Amaranthus albus atypical

MIRL22-A.unknown-43

Amaranthus hypochondriacus

1.80

Amaranthus cruentus5

MIRL22-A.unknown-44

Amaranthus hybridus

2.42

Amaranthus hybridus

MIRL22-A.unknown-45

Amaranthus albus

2.08

Amaranthus albus

MIRL22-A.unknown-46

Amaranthus retroflexus

2.05

Amaranthus retroflexus atypical

MIRL22-A.unknown-47

Amaranthus powelli

2.41

Amaranthus powellii subsp. bouchonii

MIRL22-A.unknown-48

Amaranthus powelli

2.23

Amaranthus powellii subsp. powellii

MIRL22-A.unknown-50

Amaranthus hypochondriacus

2.03

Amaranthus cruentus5

MIRL22-A.unknown-51

Amaranthus tuberculatus

2.17

Amaranthus tuberculatus atypical

MIRL22-A.unknown-52

Amaranthus tuberculatus

2.27

Amaranthus tuberculatus

MIRL22-A.unknown-53

Amaranthus spinosus

2.20

Amaranthus spinosus

MIRL22-A.unknown-54

Amaranthus albus

1.72

Amaranthus albus

MIRL22-A.unknown-55

Amaranthus palmeri

1.49

Amaranthus palmeri atypical

MIRL22-A.unknown-56

Amaranthus tricolor

2.22

Amaranthus tricolor

MIRL22-A.unknown-57

Amaranthus watsonii

2.00

Amaranthus palmeri5

MIRL22-A.unknown-58

Amaranthus powelli

1.92

Amaranthus powellii subsp. powellii

MIRL22-A.unknown-59

Amaranthus hybridus

2.21

Amaranthus hybridus

MIRL22-A.unknown-60

Amaranthus hypochondriacus

1.97

Amaranthus caudatus5

MIRL22-A.unknown-61

Amaranthus arenicola

2.14

Amaranthus arenicola

MIRL22-A.unknown-62

Amaranthus tricolor

2.14

Amaranthus tricolor

MIRL22-A.unknown-63

Amaranthus powelli

2.32

Amaranthus powellii subsp. bouchonii

MIRL22-A.unknown-64

Amaranthus retroflexus

2.35

Amaranthus retroflexus

MIRL22-A.unknown-65

Amaranthus retroflexus

1.97

Amaranthus retroflexus atypical

MIRL22-A.unknown-66

Amaranthus retroflexus

2.02

Amaranthus retroflexus

MIRL22-A.unknown-67

Amaranthus retroflexus

2.40

Amaranthus retroflexus

MIRL22-A.unknown-68

Flatline4

0.00

Amaranthus palmeri atypical

MIRL22-A.unknown-69

Amaranthus arenicola

2.03

Amaranthus arenicola

MIRL22-A.unknown-70

Flatline4

0.00

Amaranthus tuberculatus atypical

MIRL22-A.unknown-72

Amaranthus palmeri

2.05

Amaranthus palmeri

MIRL22-A.unknown-73

Amaranthus watsonii

2.02

Amaranthus palmeri5

MIRL22-A.unknown-74

Amaranthus hybridus

2.43

Amaranthus hybridus

MIRL22-A.unknown-75

Amaranthus powelli

1.90

Amaranthus powellii subsp. bouchonii

MIRL22-A.unknown-76

Amaranthus californicus

2.14

Amaranthus californicus

MIRL22-A.unknown-77

Amaranthus retroflexus

2.30

Amaranthus retroflexus

MIRL22-A.unknown-78

Amaranthus retroflexus

2.16

Amaranthus retroflexus

MIRL22-A.unknown-79

Amaranthus powelli

2.52

Amaranthus powellii

MIRL22-A.unknown-80

Amaranthus tricolor

2.09

Amaranthus tricolor

MIRL22-A.unknown-81

Amaranthus caudatus

2.43

Amaranthus caudatus

MIRL22-A.unknown-82

Amaranthus spinosus

2.04

Amaranthus spinosus

MIRL22-A.unknown-83

Amaranthus retroflexus

2.12

Amaranthus retroflexus

MIRL22-A.unknown-84

Amaranthus powelli

2.40

Amaranthus powellii subsp. bouchonii

MIRL22-A.unknown-85

Amaranthus albus

1.97

Amaranthus albus atypical

MIRL22-A.unknown-86

Amaranthus retroflexus

2.41

Amaranthus retroflexus

  1. 1 Each row corresponds to a single seed selected from a single source. The bioyping identification call of each one of these seeds used a majority rule for 3 technical replicates per seed (if two technical replicates indicated one species, the sample was catalogued as such). Original data with technical replicates can be found in Additional file 5
  2. 2 Average score from 3 technical replicates on the same seed. When using 2 technical replicates for the majority rule identification, the average was done between the 2 matching reps
  3. 3 Samples marked as ‘atypical’ correspond to seeds that are less mature or outside the range of variation for typical seeds and would result in uncertain identification
  4. 4 Flatlined samples did not produce a protein spectra in any of the three technical replicates
  5. 5 Mismatches between protein biotyping and phenotypic identification
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Plant Methods

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