Table 1 Details of V. mali inoculation methods
| Â | Growth conditions | Treatment | Inoculation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|  | Media | Time (day) | Temperature (℃) | Speed (rpm) | Tool | Time (min) | Speed (rpm) | Outcome (PDA/PDL) | OD600 | Method | Time (h/min) |
M1 | PDA | 3 | 25 ± 2 ℃ | 0 | Sterile tips | – | – | Mycelial plugs | – | Contact | 24 h |
M2 | PDA | 5 | 25 ± 2 ℃ | 0 | Sterile tips | – | – | Mycelial suspension | 1.6 | Soak | 10 min |
M3 | PDA + cellophane | 7 | 25 ± 2 ℃ | 0 | Glass beads | 30 | 200 | Mycelial suspension | 1.6 | Soak | 10 min |
M4 | PDL | 7 | 25 ± 2 ℃ | 0 | Glass beads | 30 | 200 | Mycelial suspension | 1.6 | Soak | 10 min |
M5 | PDL + glass beads | 7–9 | 25 ± 2 ℃ | 200 | – | – | – | Mycelial suspension | 1.6 | Soak | 10 min |