Fig. 7

Outlines of disease-resistant gene screening system of M. sieversii in response to V. mali. Three-month-old seedlings of M. sieversii were surface-sterilized with 75% ethanol and then transiently transformed with EHA105 containing pCambia1307-Flag-Gene recombined vector. The leaves and stems were then infected with V. mali using a suitable method. To investigate the incidence and lesion areas, leaves were photographed for 3 days. Then the leaves were harvested to analyze H₂O₂ content, MDA content, and the V. mal biomass. The phloem around the wounds on stems was removed after 5 days of incubation to expose the lesion length. Surface sterilized stems were cut into segments (1 cm), then placed on a PDA medium and photographed to study cultured V. mali biomass