Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus

Fig. 5

Determining function of MsERF1B, MsbHLH41 and MsEIL3 for resistance in stems against fungi pathogen. Three transiently transformed over-expressed plants (MsERF1B-OE, MsEIL3-OE, and MsbHLH41-OE) and a control line (Con) transiently transformed with pCambia1307-Flag were tested. The phenotype (A), incidence rate (B), lesion length (C), and cultivated fungal biomass (D) of 4 kinds of transiently transformed stems inoculated with V. mali. The stem samples were harvested at 5 days post-inoculation. The lesion length was measured with the ImageJ software. The cultivated fungal biomass was counted for 3 days from the surface-sterilized stem segment placed on PDA. Data are the means ± SE of three biological repeats (sample size of 6 stems). A student’s t-test was performed. *P < 0.05, **P < 0.01

Back to article page

Plant Methods

ISSN: 1746-4811