Fig. 4

GFP signals showing AtTT10 and AtDP1 promoter activities in transgenic camelina seeds. (A) Three seeds of camelina WT (‘Suneson’), transgenic camelina with AtTT10p:eGFP-GUS (labeled as TT10 in short), and transgenic camelina with AtDP1p:eGFP-GUS (labeled as DP1 in short), were arranged into a single view field of a dissecting microscope and observed under bright field. Panel B, E, F, I and J were organized the same way. (B) The same samples in panel A were observed under the GFP florescent channel. (C) One seed from each of the three genotypes shown in panel A and B was cut along the longitudinal direction with the cutting sides facing up, and observed using a dissecting microscope under bright field. Panel D, G, H, K and L were organized the same way. Arrows indicate the embryos in each dissected seed, the embryos were placed next to the seed coat tissue. (D) The same samples in panel C were observed under the GFP florescent channel. (A-D) Seeds at linear cotyledon development stage. (E-H) Seeds at the walking-stick embryo development stage. (I-L) Seeds at the green mature development stage. Field area of panel A, B, E, F, I and J are the same; field area of panel C, D, G, H, K and L are the same