Fig. 3
From: Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina
GFP signals showing AtTT10 and AtDP1 promoter activities in transgenic pennycress seeds. (A) Three seeds of pennycress WT (‘MN108’), transgenic pennycress with AtTT10p:eGFP-GUS (labeled as TT10 in short), and transgenic pennycress with AtDP1p:eGFP-GUS (labeled as DP1 in short), were arranged into a single view field of a dissecting microscope and observed under bright field. Panel B, E, F, I and J were organized the same way. (B) The same samples in panel A were observed under the GFP florescent channel. (C) One seed from each of the three genotypes shown in panel A and B was cut along the longitudinal direction with the cutting sides facing up, and observed using a dissecting microscope under bright field. Panel D, G, H, K and L were organized the same way. Arrows indicate the embryos in each dissected seed. (D) The same samples in panel C were observed under the GFP florescent channel. (A-D) Seeds at linear cotyledon development stage. (E-H) Seeds at the walking-stick embryo development stage. (I-L) Seeds at the green mature development stage. Field area of panel A, B, E, F, I and J are the same; field area of panel C, D, G, H, K and L are the same