Fig. 4
Effects of NEM, BDM, and DDG on sugars transport in SAM of Siberia bulblets. a, b Simulation of symplastic transport by CFDA in central buds of NEM, BDM, and DDG-treated bulblets (a). Compared to the control (ddH2O), CFDA was less expanded to the surrounding parenchyma cells under NEM treatment, while BDM and DDG promoted CFDA expansion (b). The green fluorescence of CFDA and ImageJ software were used for quantification. Scale bars, 100 μm, Vb: vascular bundles. c Soluble sugar was measured in SAMs of bulblets 6 weeks after the treatments with NEM, BDM, and DDG. The data in panels b and c represent mean ± s.d. of 3 biological replicates (n = 3 slices/SAMs per sample). Different letters indicate significant differences (p < 0.05) by ANOVA Turkey’s HSD tests for pairwise comparisons