Table 2 Confocal microscopy image acquisition settings

Figure 2a–d

Blue

405 nm (diode laser)

425–480 nm

50 slices with step size of 2 µm (Fig. 2a–c); none (Fig. 2d)

Green

499 nm

505–550 nm

Red

557 nm

565–610 nm

Figures 2e and 4

Blue

405 nm (diode laser)

425–480 nm

20 slices with step size of 4 µm (Fig. 2e); 25 slices with step size of 2 µm (Fig. 4)

The brightness and contrast were adjusted by FIJI

Green

488 nm

495–525 nm

Yellow

520 nm

535–565 nm

Red

557 nm

565–610 nm

Figure 3

Blue

405 nm (diode laser)

425–480 nm

30 slices with step size of 2 µm

The brightness and contrast of green channel were adjusted by FIJI in the same way across panels

Green

488 nm

495–535 nm

Red

557 nm

565–610 nm

Figure 5

Grey

405 nm

419–800 nm

none

Monocot cell wall autofluorescence

Magenta

635 nm

649–791 nm

none

The brightness and contrast were adjusted by FIJI