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Fig. 5 | Plant Methods

Fig. 5

From: A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol

Fig. 5

HCR RNA-FISH in monocot roots. HCR RNA-FISH signal in magenta, and cell wall autofluorescence in gray, in either maize (a–c) or sorghum (d–f) roots. a,d FISH background fluorescence was assessed using RNA-FISH for eGFP transcripts, not expressed in the roots. b,c longitudinal hand sectioning of maize root tips, revealing the signal for FISH against GRP4 or SCR1h maize genes, specifically expressed in lateral root cap/epidermis (b) or endodermis (c). (e) transversal and longitudinal (f) hand section of sorghum root, RNA-FISH for SORBI-3003G296400 specifically expressed in xylem (e) or for SORBI-3003G079300 expressed in endodermis (f)

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Plant Methods

ISSN: 1746-4811