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Fig. 3 | Plant Methods

Fig. 3

From: A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol

Fig. 3

HCR RNA-FISH in the inflorescence of Arabidopsis transgenic reporter. EGFP fluorescence (green) and RNA-FISH for EGFP transcripts (red) were detected in gTFL1-GFP tfl1-1 (a), Col-0 wild type (b) (top-view with maximum intensity projection). The detection in Col-0 wild type served as a negative control. c EGFP fluorescence (green) and FISH for EGFP transcripts (red) in gTFL1-GFP tfl1-1 with proteinase K treatment (top-view with maximum intensity projection). Nuclei were stained by DAPI (blue). Scale bar = 100 µm

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Plant Methods

ISSN: 1746-4811