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Fig. 1 | Plant Methods

Fig. 1

From: A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol

Fig. 1

Schematic of plant wholemount HCR RNA-FISH workflow and mechanism. a Timeline of 3-day plant wholemount HCR RNA-FISH protocol. b In the hybridization stage, HCR hybridization probes anneal to the target RNA, and adjacent probes form an initiator which allows the initiation of the HCR amplification. c In the amplification stage, HCR hairpins (h1 and h2) stay self-annealing in the absence of an initiator. When an initiator is present, hairpin h1 and hairpin h2 hybridize to each other and initiate the self-assembly (hybridization chain reaction). Hairpins are tagged by fluorescent dyes (green star), and the self-assembly leads to an amplification of fluorescent signals

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Plant Methods

ISSN: 1746-4811