Table 3 Application, advantages, and disadvantages of molecular techniques with antibody-based probes in AGPs studies
Technique | Application | Advantage | Disadvantage |
|---|---|---|---|
Western blotting | Used to detect the molecular weight of AGPs | Separation of proteins according to molecular weight High specificity and sensitivity Nice and clean interpretable results Commercially available antibodies | Work-intensive and not quick Medium throughput High cost and technical demand Tissue must be homogenised Low intensity of bands can distort the results |
ELISA | Used to detect AGPs qualitatively and quantitatively | High specificity, sensitivity, and efficiency Easy to perform with a simple procedure Commercially available reagents for detection Simultaneous analysis of several samples | High cost Insufficient blocking of immobilised antigens yields false results Results limited to the amount of the antigen in the sample |