Table 1 Characterisation of AGP epitopes recognized by specific antibodies
Antibody name | Epitope structure | Characterisation | M.W. of Protein Antigen (kDa) | References |
|---|---|---|---|---|
JIM4 | β-GlcA(1 → 3)-α-GalA(1 → 2)-Rha | Recognises oligosaccharides; binds to a set of discreet protein bands | 70–100 | |
JIM8 | Carbohydrate portion of arabinogalactan proteins | Binds to a galactose-rich epitope of agps; the epitope contains one or more galactose residues | 68, 84, 160 | |
JIM13 | β-GlcA(1 → 3)-α-GalA(1 → 2)-α-Rha | It is most likely not the complete epitope structure, because it binds to other anti-AGP glycan antibodies | 80–100 | |
JIM14 | β-Gal(1 → 6)-β-Gal(1 → 6)-β-Gal(1 → 6) | Binds to at least three consecutive galactans with β-1,6-linked galactan; is used to visualise differentially branched galactan in type II AG polysaccharides | 80–100 | |
JIM15 | D-GlcA, GlcA-β(1-O-Me) | Recognises an epitope distinct from JIM13 and JIM14 but uncharacterised | 80–100 | |
JIM16 | β-Gal(1 → 3)-β-Gal(1 → 3)-β- Gal(1 → 3) | Binds to a β-1,3-linked galactan backbone when substituted with a single β-1,6-linked Gal residue; used to visualise differentially branched galactan in type II AG | 80–100 | |
JIM101 | unknown | Binds strongly to okra rhamnogalacturonan I and seed mucilages from Sinapus alba and Camelina sativa | No information | [31] |
JIM133 | β-1,3-linked galactooligosaccharides | Detects the nonreducing ends of the β-1,3-linked galactan backbone in the AG structures of agps | No information | |
MAC204 | Arabinogalactan (epitope structure unknown) | Binds to arabinogalactan proteins, but the epitope has not been characterised in detail | 90–100 | |
MAC207 | β-GlcA(1 → 3)-α-GalA(1 → 2)-Rha | Recognises an arabinose-containing epitope | 70–100 | |
LM2 | β-D-GlcA | Recognises a carbohydrate epitope containing β-linked D-glucuronic acid in AGP glycan | No information | |
LM14 | AG type II arabinogalactan | Recognises arabinose and galactose-rich epitopes | No information | [34] |
LM30 | Arabinogalactan | Binds to terminal arabinose residues linked to galactan | No information | [35] |
PN 16.4B4 | Uncharacterised epitope in the carbohydrate part of the glycoprotein | Binds to arabinogalactan glycoproteins | 135–180 |