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Fig. 4 | Plant Methods

Fig. 4

From: A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Fig. 4

Scheme of measuring AGPs using Western Blotting. (1) Homogenisation of plant material in liquid nitrogen. (2) Extraction step. (3) Collection of the supernatant. (4) Application of samples and protein leader to electrophoresis gel. (5) SDS-PAGE electrophoretic separation. (6) Preparation of the sandwich. Membrane transfer. (7) Membrane blocking with BSA and incubation with primary antibody. (8) Membrane incubation with secondary antibody. (9) Signal detection and band measurement. (10) Imaging with a scanning machine. Created with BioRender.com

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Plant Methods

ISSN: 1746-4811