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Fig. 1 | Plant Methods

Fig. 1

From: A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Fig. 1

Scheme of the preparative sequence of microscopic methods. (1) Preparation, fixation, and encapsulation of plant material. (2) Polymerisation of capsules with fixed plant material. (3) Cutting capsules into semi-thin and ultra-thin sections. (4) Mounting the sections on poly-L-lysine-coated glass slices (for CLSM) or nickel grids (for TEM). (5) Immunofluorescence labelling – method on poly-l-lysine-coated slices. (6) Imaging with CLSM. (7) Immunogold labelling – method on grids with formvar film (8) Imaging with TEM. Created with BioRender.com

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Plant Methods

ISSN: 1746-4811