Table 2 PCR1 and PCR2 primer specifications
From: Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples
Primer | Sequence |
|---|---|
PCR1 | |
rbcL1 (F) | AGACCTWTTTGAAGAAGGTTCWGT |
rbcL1 (R) | TCGCATGTACCTGCAGTAGC |
ITS2 (F) | ATGCGATACTTGGTGTGAAT |
ITS2 (R) | TCCTCCGCTTATTGATATGC |
PCR2 | |
rbcL1 (F) | CAGCGTCAGATGTGTATAAGAGACAGAGACCTWTTTGAAGAAGGTTCWGT |
rbcL1 (R) | GCTCGGAGATGTGTATAAGAGACAGTCGCATGTACCTGCAGTAGC |
ITS2 (F) | CAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT |
ITS2 (R) | GCTCGGAGATGTGTATAAGAGACAGTCCTCCGCTTATTGATATGC |