Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples

Table 1 Linear models fitted to predict melissopalynological data from metagenetic data, and related correlation tests

Taxonomic level	Marker	Adj. R²	RSE	p-value	Pearson’s r	Spearman’s ρ
Family	ITS2	0.289	25.14	< 0.001	0.544***	0.724***
	rbcL1	0.730	15.49	< 0.001	0.856***	0.816***
	Average	0.626	18.24	< 0.001	0.793***	0.830***
Genus	ITS2	0.311	16.26	< 0.001	0.560***	0.585***
	rbcL1	0.410	15.05	< 0.001	0.642***	0.611***
	Average	0.447	14.57	< 0.001	0.670 ***	0.659***

Linear models fitted to predict melissopalynological proportional data from paired metagenetic proportional data. Adj. R² is the adjusted model fit statistic for each respective linear model, RSE is the residual standard error. Pearson’s r is the product-moment correlation coefficient, Spearman’s ρ is the rank-based correlation coefficient
***Indicates a statistically significant relationship (p < 0.001). Average is the multi-locus average across both primers

ISSN: 1746-4811