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Fig. 4 | Plant Methods

Fig. 4

From: Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflows

Fig. 4

The SQANTI3 and coding potential analyses for the four Apetala2 genes obtained from jojoba (Simmondsia chinensis) reference genome. a isoforms count frequency per library size type of standard and long. b distribution of SQANTI3 structural classification including incomplete full-splice match (ISM), novel in catalog (NIC), novel not in catalog (NNIC), intergenic, and genic isoforms. c isoforms count frequency for the four Apetala2 genes by their length in base pair (bp). d distribution of SQANTI3 canonical and non-canonical isoforms for the known and novel genes for all the four apetala genes plus the number of the completely novel isoforms with unknown sequence identification. e the coding potential for the four Apetala2 genes isoforms obtained from the jojoba (Simmondsia chinensis) reference genome and Iso-Seq reference. The coding potential including the four categories: complete, 5’ potential, 3’ potential, and noncoding sequences. f sub-category classification for the four Apetala2 genes including intron retention (IR), 5 prime fragment, 3 prime fragment, alternative 3 end 5 end, and at least one novel splice site

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