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Fig. 3 | Plant Methods

Fig. 3

From: Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflows

Fig. 3

Comparison of PacBio Iso-Seq standard and long workflow derived transcripts isoform sequences using four genes with annotated coding sequences above 5,000 bp. PacBio isoforms were generated using the standard workflow (SW) and the long workflow (LW). Circular consensus read redundancy in both datasets was removed using high-quality (HQ) isoforms post Cd-Hit at 0.99% before combining to then generate SW + LW. Unique LW-derived transcript isoform/s are present if the black bar is higher than the blue bar in any specific isoform size range or if absence of a blue bar and presence of an orange bar. Number and length distribution of transcript isoforms derived from the SW, LW and SW + LF mapped to four genes are shown. LW-derived unique isoforms were generated for all four genes. Mapping was undertaken using the “enable long-read spliced alignment” option in Minimap2 and executed via the CLC Genomics Workbench

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Plant Methods

ISSN: 1746-4811