Fig. 2

Comparison of PacBio Iso-Seq standard and long workflows derived transcripts isoform sequences using the apetala genes with annotated coding sequences under 5000 bp. PacBio isoforms were generated using the standard workflow (SW) and the long workflow (LW). Circular consensus read redundancy in both datasets was removed using high quality high-quality (HQ) isoforms post Cd-Hit at 0.99% before combining to then generate SW + LW. Unique LW-derived transcript isoform/s are present if the black bar is higher than the blue bar in any specific isoform size range or if absence of a blue bar and presence of an orange bar. Number and length distribution of transcript isoforms derived from the SW, LW and SW + LF mapped to four apetala genes are shown. The HQ isoforms post Cd-Hit data indicates similar sequence in both workflows Transcript isoforms except for one unique isoform for Apetala2-1 gene at the size range 3001 bp to 4000 bp. Mapping was undertaken using the “enable long-read spliced alignment” option in Minimap2 and executed via the CLC Genomics Workbench