Fig. 1

Comparison between isoforms transcripts obtained from standard and long datasets. a size distribution of transcript isoforms from three high-quality (HQ) isoforms posts Cd-Hit different datasets: standard (SW) (light blue), long (LW) (pink), and merged dataset (MW) (green) derived from jojoba (Simmondsia chinensis) transcriptome Iso-Seq reference. b distribution of unique isoforms transcripts from the long workflow across genes with CDS length of over five thousand base pairs. Out of a total of 41,539 full length CDS sequences [15], 201 CDS had length over 5000 bp and of these 196 had at least one mapped transcript isoform. For the 196 CDS sequences, a total of 4,969 common transcript isoforms between SW and LW were mapped with 2388 unique transcripts generated by the LW. Across all the isoforms, the average ratio of unique isoforms generated by LW/total isoforms by the SW generated isoforms was 1.03. Common transcript isoforms derived from SW and LW mapped to each of the 196 CDS are shown as blue bars and had a percentage of 24.9%, the unique transcripts isoforms derived from the SW are shown as black bars and had a percentage of 27.0%, while the unique transcripts isoforms derived from the LW and shown as orange bars had a percentage of 48.1%. Transcript isoforms shown here are Cd-Hit CCS sequences at 99% similarity. Mapping was undertaken using the “enable long-read spliced alignment” option in Minimap2 and executed via the CLC Genomics Workbench. This data contrasts with the previous report of Illumina short transcripts assembled to construct a jojoba reference transcriptome [16]