Table 2 Overview of derivatization reagents used in plant hormone analysis
From: Can plant hormonomics be built on simple analysis? A review
Target analyte | Reagent | Reaction conditions | Analysis type | References |
|---|---|---|---|---|
Carboxy group | Diazomethane | Room temp | LC–MS, GC–MS | |
Carboxy group | Bromocholine | 80 °C, 130 min | LC–MS | [107] |
BRs (vicinal diol) | 3-(Dimethylamino)-phenylboronic acid (DMAPBA) | 40 °C, 60 min | LC–MS | [188] |
Carboxy group (GBs) | N,N-Dimethyl ethylene diamine (DMED) and D4-DMED | 40 °C, 60 min | LC–MS | [194] |
BRs (vicinal diol) | 4-Phenylaminomethyl-benzeneboronic acid (4-PAMBA) and D5-4-PAMBA | Room temp | LC–MS | [144] |
BRs (vicinal diol) | 2-Methyl-4-phenylaminomethyl-benzeneboronic acid (2-methyl-PAMBA) | 40 °C,10 min | LC–MS | [132] |
IAAld + IPyA | Cysteamine | Room temp, 15 min | LC–MS | |
IAAld + IPyA | Methoxyamine | During extraction | LC–MS | [120] |
BRs (vicinal diol) | 2-Methoxypyridine-5-boronic acid (MpyBA) | 40 °C, 60 min | LC–MS | |
Carboxy group | N,N-Diethyl ethylene diamine (DEED) | 40 °C, 10 min | LC–MS | [132] |
ACC | Phenyl isothiocyanate (PITC) | (1) room temp, (2) 20 min 40% TFA, 90 °C, 60 min | LC–MS | [206] |
ACC | 9-Fluorenylmethyloxycarbonyl chloride | Room temp, 5 min | LC–MS | |
ACC | Pentafluorobenzyl bromide | 60 °C, 15 min | GC–MS | [207] |
ACC | Marfey’s reagent | 37 °C 120 min | LC–MS | [210] |
ACC | Methoxyamine + N-methyl-Ntrimethylsilyl trifluoroacetamide | (1) 90 °C 90 min, (2) 90 °C 60 min | GC–MS | [211] |