Fig. 7

Reflectance spectra of irCHAL differed significantly from the reference genotype (EV) at the beginning of the VIS in the field, and the irAOC line differed significantly from EV in infrared wavelengths in the glasshouse, corresponding to generally greater variation among the TLs in the infrared in the glasshouse, but not in the field. a TLs compared with EV in mixed models in Field_UT. Each dashed colored line indicates the p-value of the corresponding TL—green: irCHAL, vermilion: irLOX3 – compared with EV in the mixed model, without adjustment for testing multiple wavelengths. The solid colored line shows the adjusted p-value of the TL versus EV. All other comparisons of EV with transgenics become 1 across all wavelengths after adjustment and can be found in Additional file 1: Fig. S4. (b) The coefficient of variation (CV) of TL and EV samples in Field_UT. The blue shaded area indicates the range of bootstrapped (100 times) CV results for TLs and the dashed blue line indicates the mean of the bootstrapped results. The black line shows the CV of the EV samples. (c) TLs compared with EV in the linear model in the Glasshouse. Each dashed colored line indicates the p-value of the corresponding TL—blue: irAOC, orange: irRCA—compared with EV in the linear model without adjustment. The solid colored line shows the adjusted p-values. d The coefficient of variation (CV) of TLs and EVs samples in the Glasshouse. Interpretations of colors and lines are the same as in (b)