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Fig. 7 | Plant Methods

Fig. 7

From: A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation

Fig. 7

Enhancement of thermotolerance in E. coli as a result of the presence of farnesylated AtJ3. a E.coli strains harboring pGEX-4T-1 and pRSF-Duet1 empty vectors (contains no AtJ3), pGEX-AtJ3 and pRSF-Duet1 empty vector (contains unfarnesylated AtJ3), and both pGEX-AtJ3 and pRSF-Duet1-AtPFT (contains farnesylated AtJ3) were induced with IPTG then heat stressed at 52 °C for 5 min. After the treatment, ten-fold dilutions of E. coli cells were spotted on LB plates and incubated at 37 °C for 16 h before pictures were taken. b The survival rate of E. coli cells containing farnesylated AtJ3 was 65.2% compared to 39.8% for cells containing unfarnesylated AtJ3 and 33.7% of those containing no AtJ3. Values represent the mean ± SD of three biological replicates. *P ≤ 0.01 (unpaired t-test). c E. coli cells either co-expressed with nYFP-DnaJ and cYFP-DnaK or nYFT-AtJ3 and cYFP-DnaK exhibited BiFC fluorescence, indicating AtJ3 can interact with DnaK in E. coli. Bar = 3 μm

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Plant Methods

ISSN: 1746-4811