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Fig. 4 | Plant Methods

Fig. 4

From: A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation

Fig. 4

Co-expression of OsPFTα, OsPFTβ, and OsDjA4 in E. coli. a Coomassie blue staining of a gel with proteins extracted from E. coli cells harboring either pGEX-OsDjA4 alone or both pGEX-OsDjA4 and pRSF-Duet1-OsPFT, before or after IPTG induction. b The protein samples in (a) were analyzed using western blot analysis with an anti-6xHis-tag antibody. The molecular size of GST-6xHis-OsDjA4 (73.11 kDa, red arrowhead), 6xHis-OsPFTβ (53.43 kDa, green arrowhead), and 6xHis-AtPFTα (38.2 kDa, orange arrowhead) are indicated

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Plant Methods

ISSN: 1746-4811