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Fig. 2 | Plant Methods

Fig. 2

From: A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation

Fig. 2

Co-expression of AtPFTα, AtPFTβ, and AtJ3 in E. coli. a Coomassie blue staining of a gel with proteins extracted from E. coli cells containing either pGEX-AtJ3 alone or both pGEX-AtJ3 and pRSF-Duet1-AtPFT before or after IPTG induction. b The protein samples in (a) were analyzed using western blot analysis with anti-6xHis-tag antibody. The molecular size of GST-6xHis-AtJ3 (72.67 kDa, red arrowhead), 6xHis-AtPFTβ (54.99 kDa, green arrowhead), and 6xHis-AtPFTα (38.78 kDa, orange arrowhead) are indicated

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Plant Methods

ISSN: 1746-4811