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Fig. 1 | Plant Methods

Fig. 1

From: A plant protein farnesylation system in prokaryotic cells reveals Arabidopsis AtJ3 produced and farnesylated in E. coli maintains its function of protecting proteins from heat inactivation

Fig. 1

Schematic diagram illustrating the structure of vectors for plant protein farnesylation system in E. coli. a Arabidopsis AtPFTα and AtPFTβ cDNAs were cloned into multiple cloning sites 1 (MCS-1) and 2 (MCS-2) of pRSF-Duet1, respectively, to generate pRSF-Duet1-AtPFT. Both AtPFTα and AtPFTβ cDNA were fused with a 6x His-tag sequence downstream of a lac operator. Arabidopsis AtJ3 cDNA was cloned into expression vector pGEX-4T-1 to generate pGEX-AtJ3. In pGEX-AtJ3, AtJ3 was fused with both a GST and a 6x His-tag sequence for affinity purification and immunoblot identification. AtJ3 cDNA was also downstream of a lac operator for ITPG induction. b Rice OsPFTα and OsPFTβ cDNA were cloned into MCS-1 and MCS-2 of pRSF-Duet1 to generate pRSF-Duet1-OsPFT; rice OsDjA4 cDNA was cloned into pGEX-4T-1 to generate pGEX-OsDjA4.

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Plant Methods

ISSN: 1746-4811