Table 3 Summary of PCR and Sequencing results for each DNA isolation method per species and barcode marker
From: Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency
| Â | Â | PCR | Sequencing | ||||||
|---|---|---|---|---|---|---|---|---|---|
rbcL | trnH-psbA | rbcL | trnH-psbA | ||||||
DNA isolation Method | Species | N | Major amplicon detected % | Minor bands detected % | Major amplicon detected % | Minor bands detected % | N | Expected seq. length % | Expected seq. length % |
QIAGEN | TP | 4 | 100 | 50 | 100 | 0 | 2 | 100 | 100 |
PM | 100 | 50 | 100 | 0 | 50 | 100 | |||
TH | 100 | 0 | 100 | 0 | 100 | 100 | |||
MicroGEM | TP | 100 | 75 | 100 | 0 | 50 | 100 | ||
PM | 100 | 100 | 100 | 0 | 50 | 100 | |||
TH | 100 | 50 | 100 | 0 | 100 | 100 | |||