Table 2 Shoot induction in different growth hormone combinations
From: Plant regeneration through somatic embryogenesis in cell suspensions of Cenchrus ciliaris L.
Media combination | Response | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
S. no. | MS | BAP mg l−1 | 2,4-D mg l−1 | TDZ mg l−1 | IAA mg l−1 | Kinetin mg l−1 | Amino acids | Total no. of embryos formed | Total no. of shoots formed after 3 weeks | Total no. of shoots formed after 5 weeks |
1 | Basal MS | – | – | – | – | – | – | – | – | – |
2 | Basal MS | 1 | 0.5 | – | – | – | – | 26.33 ± 2.18de | 1.00 ± 0.00c | 2.34 ± 1.20c |
3 | Basal MS | 1 | 0.5 | – | – | – | CGP* | 39.31 ± 2.84c | 4.00 ± 1.15b | 9.33 ± 0.88b |
4 | Basal MS | 2 | 0.5 | – | – | – | – | 14.00 ± 2.08f | – | – |
5 | Basal MS | 3 | 0.5 | – | – | – | – | 6.66 ± 0.88gh | – | – |
6 | Basal MS | 4 | 0.5 | – | – | – | – | – | – | – |
7 | Basal MS | 5 | 0.5 | – | – | – | – | – | – | – |
8 | Basal MS | 1 | 1 | – | – | – | CGP* | 66.00 ± 4.04a | 8.00 ± 1.15a | 16.00 ± 3.78a |
9 | Basal MS | 1 | – | 0.1 | 1 | – | – | 20.62 ± 1.45e | – | – |
10 | Basal MS | 1 | – | 0.1 | – | – | – | 25.34 ± 2.72de | – | – |
11 | Basal MS | 1 | – | – | – | – | – | – | – | – |
12 | Basal MS | 0.1 | – | 0.1 | – | – | 31.00 ± 2.30d | – | 2.00 ± 1.15c | |
13 | Basal MS | 0.1 | – | 0.1 | 1 | – | – | 50.65 ± 4.40b | 3.00 ± 0.58b | 11.00 ± 2.30b |
14 | Basal MS | 3 | 0.25 | – | 1 | – | – | 21.29 ± 3.75e | – | – |
15 | Basal MS | 3 | 0.25 | – | – | 0.1 | – | 11.00 ± 1.73fg | – | – |
16 | Basal MS | 3 | – | – | 1 | 1 | – | 8.66 ± 1.45fg | – | – |
17 | Basal MS | – | – | – | 1 | 0.1 | – | – | – | – |