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Table 7 Stock solutions of fluorochromes used for protoplast labelling and cellulose detection

From: Early selection of carrot somatic hybrids: a promising tool for species with high regenerative ability

Fluorochrome

Solvent

Concentration (%)

Fluorescein diacetate (FDA)

Acetone

0.5

Rhodamine B isothiocyanate (RBITC)

dH2O

0.5

Scopoletin

0.6 M mannitol (pH = 9.0)

0.1

Calcofluor white (CW)

NaOH + dH2O (pH = 10–11)

1.0

  1. All stock solutions were filter-sterilized (0.22 µm, Millipore) and stored in aliquots in – 20 ℃
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Plant Methods

ISSN: 1746-4811