Early selection of carrot somatic hybrids: a promising tool for species with high regenerative ability

Table 4 Effect of direct current (DC) pulse duration on hetero-fusion frequency between D. carota subsp. sativus cv. Dolanka and D. carota subsp. gadecaei protoplasts

Duration (µs) of 2 kV cm⁻¹ DC	The frequency of hetero-fusion events (%)
Duration (µs) of 2 kV cm⁻¹ DC	Micro-fusion chamber	Macro-fusion chamber
70	19.3 ± 0.5 a	5.3 ± 0.6 b
80	15.9 ± 0.8 a	3.0 ± 0.1 c
90	21.6 ± 4.7 a	8.2 ± 0.5 a
Average	18.9 ± 1.6	5.5 ± 0.8

Data represent means and standard errors calculated from three independent fusion experiments per each fusion variant. Means within columns followed by the same letter were not significantly different at least at P = 0.05

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