Early selection of carrot somatic hybrids: a promising tool for species with high regenerative ability

Table 1 The effect of feeder layer volume on development of manually selected leaf-derived protoplasts in two Daucus accessions

Source of manually selected protoplasts	Volume of feeder layer (µl)^a	Mitotic divisions^b of manually selected protoplasts in		Somatic embryos^c
Source of manually selected protoplasts	Volume of feeder layer (µl)^a	10-day-old cultures	20-day-old cultures	Somatic embryos^c
cv. Dolanka	0	–	−	−
	100	±	−	−
	200	+	+ +	+
D. carota subsp. gadecaei	0	−	−	−
	100	±	−	−
	200	+	+ +	+

^aSource of nurse protoplasts for feeder layer preparation was the same as for manual selection; feeder layer was formed from protoplast-alginate suspension with final protoplast density of 4 × 10⁵ per ml;
^b(−) Cell divisions not observed; ( ±) occasional cell divisions, unrepeatable in subsequent repetitions; ( +) repeatable cell divisions of individual cells; (+ +) from manually selected protoplasts of proper morphology and undamaged membrane at least 50% re-entered mitotic divisions and cell aggregates were formed;
^c(−) Not occurred; ( +) occurred; three independent experiments per each variant have been performed; in a single experiment 100 protoplasts per variant were manually selected and embedded in four alginate droplets (25 protoplasts per droplet)

ISSN: 1746-4811