Fig. 6

Amplification of DcS-ILP markers differentiating donors and identifying somatic hybrids of cv. Dolanka ( +) Daucus carota subsp. gadecaei. Data shown for five randomly chosen plants of cv. Dolanka (marked with a green bracket), Daucus carota subsp. gadecaei (D.c.g.; red bracket) and putative somatic hybrids (‘Dolanka’ ( +) D.c.g.; blue bracket). A Amplification of DcS-ILP225 characterized by the presence of ca. 1470 bp and 1730 bp specific products for ‘Dolanka’, ca. 2420 bp products for D.c.g., and both specific products for hybrid plants. B Amplification of DcS-ILP516 characterized by the presence of ca. 1180 bp specific product for ‘Dolanka’ and ca. 900 bp specific products for D.c.g. Hybrids were characterised by the presence of both specific products. One putative hybrid regenerated in vitro (Dx7-1) showed only one product specific for ‘Dolanka’ donor plant (marked by yellow arrow). I—GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific^™); II—GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific^™)