Fig. 4

Protoplast-to-plant regeneration of ‘Dolanka’ ( +) D. carota subsp. gadecaei hybrids. (A–D) hybrid cells (A) emitting green (B) and red (C) fluorescence (marked by arrows); (E–H) development of a multicellular fusant-derived aggregate; (I–M) formation and development of somatic embryos observed in microcallus cultures derived from selected hybrid cells. Scale bars: 100 µm (A–H); 0.5 mm (I-L); 1 mm (M)