Fig. 2

Effect of fluorochromes on development of ‘Dolanka’ protoplast cultures. (I) A, B) fresh isolated protoplast with completely removed cell wall, (C, D) cell with partially and (E, F) completely reconstituted cell wall; (A, C, E) calcofluor-stained cells and (B, D, F) their image under bright-field illumination. (II) Frequency of cells with completely reconstructed cell walls examined after calcofluor staining of cellulose in 48 h-old cultures. (III) Plating efficiency in 10- (A) and 20-day-old (B) protoplast cultures. FDA fluorescein diacetate, RBITC rhodamine B isothiocyanate. Bars represent means ± SE obtained from three independent experiments. Statistically significant differences between variants were not observed both within particular categories of cell wall reconstitution (II) and within subsequent time points of plating efficiency (III). Scale bar: 20 µm