Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Table 1 Effects of different types of A. rhizogenes strains on hairy root transformation of woodland strawberry

Strains	Number of explant cultured¹	Number of explants with callus	Number of explants with hairy roots	Number of explants with eGFP-positive root	Induction rate of callus (%)	Regeneration rate of root (%)	Frequency of eGFP- positive root² (%)
Control³	45	5.33 ± 1.03	8.67 ± 1.15	0.00 ± 0.00	12.08 ± 2.53^a4	20.20 ± 6.01^a	0.00 ± 0.00^a
Ar1193	53	34.67 ± 2.88	21.00 ± 3.46	0.00 ± 0.00	66.17 ± 10.90^b	40.20 ± 8.94^b	0.00 ± 0.00^a
K599	47	32.67 ± 3.14	23.33 ± 3.21	0.67 ± 0.58	69.68 ± 9.37^b	49.67 ± 9.37^c	2.74 ± 2.42^b
MSU440	48	34.33 ± 7.45	25.33 ± 4.04	11.33 ± 1.15	70.81 ± 7.26^b	53.36 ± 9.41^c	45.21 ± 6.27^c
C58C1	55	37.33 ± 10.48	23.67 ± 6.65	10.67 ± 3.51	66.27 ± 10.16^b	42.91 ± 9.69^b	44.68 ± 5.57^c

1. Infection experiment was repeated three times, hypocotyls from seedling at seven days after germination were used as explants
2. (Number of explants with GFP-positive roots/Number of explants with hairy root) × 100%
3. MS suspension-infested hypocotyls were used as a negative control
4. Means with different letters indicate significant differences (ANOVA test, p < 0.01)

ISSN: 1746-4811