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Fig. 4 | Plant Methods

Fig. 4

From: Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Fig. 4

Phenotype analysis and PCR verification of A. rhizogenesmediated hairy root transformation of woodland strawberry. A. Transgenic roots and corresponding eGFP fluorescence from wounded hypocotyl and SC at 15 dpi and 60 dpi. (A) rhizogenes MSU440 loading with 35S::eGFP. Hy: Hypocotyl; SC: Seedling cutoff root (including cotyledon and hypocotyl); Bar = 1 mm. (B) PCR analysis for eGFP and HPTII in five independent transgenic roots. M, 2-kb DNA ladder marker; P: 35S::eGFP plasmid DNA; YW, non-transgenic Yellow wonder DNA (negative control); T1–5, five independent transgenic hairy roots

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Plant Methods

ISSN: 1746-4811