Fig. 2

Efficiency of different explants on hairy root transformation of woodland strawberry. (A) Five types of explants, including Co, Hy, SC, Le, and Pe, were examined to compare the effects of explant types on the hairy root transformation of woodland strawberry. (B) The callus and transgenic roots induction of the five explant types. The matured seeds grow to about 6 cm in height in a 0.7% agar medium. Five kinds of explants were observed on 0, 15, and 40 dpi. eGFP fluorescence on 40 dpi with A. rhizogenes strain MSU440 was detected. The induction rates of callus (C), root regeneration rate (D), and eGFP positive rate (E) of five types of explants were compared. ***indicate significant differences (ANOVA followed using Turkey’s test, P < 0.01). Co: Cotyledon; Hy: Hypocotyl; SC: Seedling cutoff root, including cotyledon and hypocotyl; Le: Leaf; Pe: Petiole