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Fig. 3 | Plant Methods

Fig. 3

From: Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvation

Fig. 3

Faster sporophyte development on nitrogen-free medium. a Sporophyte developmental orders of 18 gametophores from a single protonemal tissue after 6 weeks of growth on the second medium. The leaves are removed to expose the gametangia or sporophytes. The media used are indicated on each panel (from the first medium to the second medium). Dashed lines indicate the border between the sporophyte developmental stages S2 and S3. b Dot plot of the ratio of intermediate and mature sporophytes (stages S3 or S3M) to the total number of gametophores (data from five tissues). Different lowercase letters represent statistically significant differences (P < 0.05; Tukey’s HSD)

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Plant Methods

ISSN: 1746-4811