Fig. 2From: Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvationEffects of nitrogen sources and their concentrations on sporophyte formation. a Schematic visual explanations of the method used for sporophyte induction in this study. b Magnified images of tissues containing gametophores with or without sporophytes at 8 weeks after transfer onto the second medium. Scales are the same between panels. c Dot plot of the formation rate of brownish mature sporophytes per tissue (the number of gametophores with mature sporophytes/total number of gametophores). The results of five tissues in each condition are shown. Different lowercase letters represent statistically significant differences (P < 0.05; Tukey’s HSD)Back to article page