Fig. 1From: Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvationEffects of the nitrogen sources and their concentrations on gametophyte growth. a Overview of protonemata at 30 days after homogenization. b, c Magnified tissues with gametophores at 10 days (b) or 18 days (c) after transplantation. d Top view of gametophores isolated from the 18-days-old tissues grown on the respective media. e Boxplot of the number of gametophores per single tissue at 18 days after transplantation (n = 30). f Boxplot of the diameter of the top view of the gametophores isolated from the tissues, as shown in (d) at 18 days after transplantation (n = 16). Different lowercase letters represent statistically significant differences (P < 0.05; Tukey’s HSD)Back to article page