Fig. 2

Minimizing and troubleshooting contamination. A Within the “Transfer duckweed” operation type a constant (“FLOW_HOOD” indicated by arrow) can be toggled between true or false depending on the needs of a particular laboratory. The value of this constant is used to hide or display specific instruction sets when the protocol is run (e.g. code lines indicated by bifurcated arrow). An optional operation type (B “Add antibiotic to media”) generates instructions to add an appropriate amount of a defined antibiotic into the media and to add a metadata tag to the media item that antibiotic has been added. As shown in panel (C), duckweed containers found to be contaminated can be entered as inputs to an operation (“Log Contamination”, blue rectangle) that, when run, will prompt the technician to upload an image (screenshot of technician interface to the right) as well as additional notes and then discard the plate. This operation produces no output item (arrow above operation) but does lead to data associations being added to the relevant item in the Aquarium database, including an entry that can be used to pull the relevant image file using a Python data analysis script