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Fig. 3 | Plant Methods

Fig. 3

From: Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Fig. 3

The utility of CGMMV-VIGS in ridge gourd using TEN gene. A The pV190-TEN vector map used in this study. B Structure diagram of cucumber and Luffa TEN gene; amino acid alignment of the TEN partial sequence of ridge gourd cloned for silencing; and the sequence of cucumber TEN gene. C Phenotype of plants with Wild type plants (WT) and infected pV190-TEN recombinant vectors at 10 and 20 dpi. D Length of tendrils at the fourth node of WT; length of tendrils at the third node of pV190-TEN recombinant vector-infected plants. E, F Length of tendrils and number of nodes of tendrils in plants with WT and infected pV190-TEN recombinant vectors at 20 dpi (due to the different nodes of each plant, the length of tendrils = the sum of the lengths of tendrils at each node/the number of nodes in the plant). G Relative expression levels of TEN genes after the pV190-TEN recombinant vector was agroinfiltrated into ridge gourd leaves. *** Stands for significant difference (P ＜0.001)

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Plant Methods

ISSN: 1746-4811