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Fig. 2 | Plant Methods

Fig. 2

From: Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Fig. 2

Silencing effect induced by CGMMV-VIGS vector in ridge gourd leaves. A The pV190-PDS vector map used in this study. B Photobleaching phenotype of ridge gourd leaves after inoculation with pV190-PDS for 22 d and 34 d. C Photobleaching phenotypes in different leaves of ridge gourd at 34 d. No. 2-1, 2-2, 2-3; 5-1, 5-2; 6-1, 6-2 represent different leaf positions on a single plant, whereas No. 1, 3 and 4 represent leaves from different plants. D Detection of pV190-PDS recombinant vector fragments after VIGS treatment [M: marker; Lane 1 is empty pV190 negative control; CGMMV RNA was detected in leaf tissues of ridge gourd that showed PDS silencing symptoms (lanes 2–12)]. E The relative expression of PDS after pV190-PDS recombinant vector was agroinfiltrated into ridge gourd leaves. Error bar indicates SE. * Stands for significant difference (P < 0.05). n = 3 biological replicates

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Plant Methods

ISSN: 1746-4811