Skip to main content
Fig. 1 | Plant Methods

Fig. 1

From: Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Fig. 1

Evaluation of TRV-VIGS in ridge gourd using PDS gene. A PDS gene structure in ridge gourds and pTRV2-PDS vector map used in this study. White boxes represent 5′- and 3′-UTRs, black boxes represent exons, and the black line represents introns, red boxes represent target silent fragment. B Agroinfiltration of ridge gourd leaves. C Detection of pTRV2-PDS recombinant vector fragments after VIGS treatment (Lane 1 is Marker; Lane 2 is empty pTRV2 negative control; TRV RNA2 was detected in the upper newly emerged leaves tissue of ridge gourds infiltrated with pTRV2-PDS Agrobacterium suspension (Lane 3–7). D RT-qPCR analysis of PDS in ridge gourd leaves of inoculated with pTRV1 and pTRV2 or inoculated with pTRV1 and pTRV2-PDS. Error bar indicates SE. * Stands for significant difference (P < 0.05). n = 3 biological replicates

Back to article page

Plant Methods

ISSN: 1746-4811