DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

Table 3 Quality and quantity of RNA extracted from leaf tissues of desert-growing P. cineraria collected in different months using the optimized protocol of the present study

Date of sample collection and temp (^oC)	A_260/280 ratio	A_260/230 ratio	RNA yield (ng/µl)
January (22)	2.17 ± 0.08	2.25 ± 0.09^a	1010.1 ± 31.6^b
March (30)	2.12 ± 0.05	2.21 ± 0.06^a	1221.5 ± 37.1^a
May (35)	2.16 ± 0.08	2.14 ± 0.07^a	997.6 ± 37.4^c
July (44)	2.10 ± 0.08	2.06 ± 0.09^ab	899.8 ± 27.6^d
September (41)	2.09 ± 0.06	2.02 ± 0.05^b	908.6 ± 32.9^d
October (38)	2.09 ± 0.04	2.17 ± 0.08^a	1000.1 ± 36.4^b
December (24)	2.12 ± 0.07	2.14 ± 0.08^a	991.8 ± 29.8^c

Data represent the mean ± SE of 3 replicates each of two biological samples. RNA dissolved in 50 µl nuclease-free water. Values followed by different letters are significantly different at the 5% level

ISSN: 1746-4811