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Table 2 Purity and yield of RNA extracted from leaves of different plants as to different protocols

From: DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

Protocol

Prosopis cineraria

Conocarpus erectus

Phoenix dactylifera

A260/280 ratio

A260/230 ratio

RNA yield (ng/µl)

A260/280 ratio

A260/230 ratio

RNA yield (ng/µl)

A260/280 ratio

A260/230 ratio

RNA yield (ng/µl)

Qiagen

1.54 ± 0.05c

1.19 ± 0.03d

26.4 ± 1.9 g

1.42 ± 0.07e

1.13 ± 0.05e.g.

19.0 ± 1.9 g

1.61 ± 0.07c

1.58 ± 0.08d

31.9 ± 2.1 g

Norgen

1.70 ± 0.08b

1.63 ± 0.07c

61.9 ± 7.2f

1.48 ± 0.09

1.30 ± 0.09f

59.5 ± 4.1f

1.75 ± 0.04b

1.72 ± 0.04c

70.1 ± 5.9f

Sigma

1.50 ± 0.09c

1.29 ± 0.08d

27.5 ± 3.2 g

1.47 ± 0.06e

1.21 ± 0.06f

23.5 ± 2.1 g

1.60 ± 0.05c

1.62 ± 0.04d

38.6 ± 3.3 g

Invitrogen

1.56 ± 0.07c

1.31 ± 0.04d

29.9 ± 4.4 g

1.51 ± 0.04d

1.19 ± 0.06 fg

56.7 ± 5.2f

1.59 ± 0.09c

1.70 ± 0.07c

36.8 ± 2.6 g

TRI Reagent Sigma

1.70 ± 0.06b

1.67 ± 0.05c

122.8 ± 6.5e

1.54 ± 0.08 cd

1.43 ± 0.03de

88.8 ± 3.5e

1.66 ± 0.07c

1.61 ± 0.06d

132.1 ± 7.1d

TRIzol Ambion

1.74 ± 0.07b

1.72 ± 0.07c

151.4 ± 5.7c

1.66 ± 0.05c

1.52 ± 0.06de

121.2 ± 7.3d

1.75 ± 0.09c

1.71 ± 0.05c

153.5 ± 5.4d

TRIzol + Column (Qiagen)

1.75 ± 0.04b

1.71 ± 0.05c

133.5 ± 5.1de

1.68 ± 0.07c

1.59 ± 0.08c

56.6 ± 2.9f

1.74 ± 0.08c

1.72 ± 0.07c

69.9 ± 4.4f

CTAB

1.98 ± 0.09a

2.05 ± 0.05b

414.2 ± 17.8b

1.91 ± 0.09b

1.89 ± 0.08b

210.9 ± 21.1b

2.00 ± 0.09a

2.09 ± 0.07a

451.8 ± 26.2b

SDS-LiCl

1.73 ± 0.05b

1.68 ± 0.06c

312.1 ± 13.9c

1.68 ± 0.06c

1.58 ± 0.04c

153.5 ± 31.0c

1.76 ± 0.06b

1.79 ± 0.07b

322.0 ± 23.5c

CTAB* Modified

2.11 ± 0.09a

2.24 ± 0.08a

1222.8 ± 32.5a

2.17 ± 0.08a

2.27 ± 0.07a

907.3 ± 25.5a

2.12 ± 0.06a

2.19 ± 0.05a

1008.6 ± 28.5a

  1. *Optimized protocol in the present study. Data represent the mean ± SE of 5 replicates. RNA dissolved in 50 µl Nuclease-free water. Values followed by different letters are significantly different at the 5% level. The samples of desert-growing P. cineraria were collected in August 2020
  2. Qiagen - RNA using RNeasy Plant Mini Kit (Cat. no. 69106; Qiagen), Invitrogen - PureLink RNA isolation kit, Sigma - Spectrum Plant Total RNA kit (Cat. no. STRN50), and Norgen- Plant/Fungi DNA/RNA isolation kit (Cat. no. 26200) CTAB – [30]; SDS-LiCl – [5]