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Fig. 6 | Plant Methods

Fig. 6

From: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Fig. 6

Combined immunoFISH-GISH detection of a somatic nucleus from a 7BS.7HL wheat-barley translocation line. A Single channel (monochrome) and merged images of a 3D-rendered nucleus. Active centromeres (CENH3 protein) are labelled with an anti-CENH3 antibody (magenta on merge), the telomeric repeat sequences (TRS) are visualised by FISH (green on merge), and the 7HL barley chromosome arm is detected by GISH (white on merge). The chromatin is counterstained with DAPI (blue on merge). B Surface rendering of the above nucleus labelled by immunoFISH-GISH. C Top row: the 3D-rendered nucleus on B is shown from two opposite sides. Bottom row: enlarged images of the 7HL barley chromosome arm (GISH, white), the active centromeres are immunolabelled (anti-CENH3, magenta), and telomeric repeat sequences (TRS) are detected by FISH (green). The chromatin is counterstained with DAPI (blue on merge). Bars = 5 μm

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Plant Methods

ISSN: 1746-4811