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Fig. 4 | Plant Methods

Fig. 4

From: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Fig. 4

Immunolabelling of barley meiotic nuclei prepared with the non-enzymatic in-solution procedure. A Single channel (monochrome) and merged images of a 3D-rendered meiotic nucleus along with two mitotic nuclei. Active centromeres are labelled by an anti-CENH3 antibody (magenta on merge) and the mono-methylated H3 histone sites labelled by an anti-H3K4me1 antibody (orange on merge). The chromatin is counterstained with DAPI (white-grey). B Immunolabelling of the synaptonemal complex transverse filament protein (ZYP1, purple) on a meiotic nucleus next to a mitotic nucleus. The chromatin is counterstained with DAPI (white). The images show the 3D-rendered meiotic nucleus from three different angles. Bars = 5 µm

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Plant Methods

ISSN: 1746-4811