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Fig. 3 | Plant Methods

Fig. 3

From: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH

Fig. 3

Immunolabelling of somatic nuclei prepared with the non-enzymatic in-solution procedure. A Single channel (monochrome) and merged images of 3D-rendered nuclei of wheat, barley and rye labelled by anti-centromeric histone H3 (anti-CENH3, magenta on merge) and anti-H3K4me1 (orange on merge) antibodies. The chromatin is counterstained with DAPI (blue on merge). B A single frame and side view of the immunolabelled (anti-H3K4me1, orange) rye nucleus presented in A (above). The chromatin is counterstained with DAPI (blue). Bars = 5 µm

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Plant Methods

ISSN: 1746-4811